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Image Search Results
Journal: Methods (San Diego, Calif.)
Article Title: Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells
doi: 10.1016/j.ymeth.2012.10.004
Figure Lengend Snippet: Correlation of qPCR and digital PCR single-cell results for transcripts from two genes. Plots show qPCR results plotted on the y -axis and digital PCR results plotted on the x -axis for CTNNB1 (A) and WNT10A (B). The results are for individual cells from 9 of the cell lines (GM07029, GM07019, GM12239, GM12864, GM12865, GM12752, GM12753, GM06991, GM11881). The qPCR values are Log 2 Ex values determined as described in Section . The digital PCR values are the log base 2 values of the number of target molecules estimated to be present in each panel of the 48.770 IFCs.
Article Snippet: For two of the assays, the preamplified cDNA samples from single cells of 9 cell lines were analyzed by digital PCR using 48.770 Dynamic
Techniques: Digital PCR